First case of glufosinate-resistant rigid ryegrass (lolium rigidum gaud.) in Greece

At the two- to three-leaf stage, the plants were sprayed with glufosinate at 0.0, 0.094, 0.187, 0.375, 0.75, 1.5, 3.0, and 6.0 kg a.i. ha−1. These rates correspond to zero, one-eighth, one-quarter, one-half, one, two, four, and eight times the recommended rate of glufosinate for rigid ryegrass. Herbicide treatments were applied as described above, with pots (12 × 13 × 5 cm) arranged in a completely randomized design. Each pot (one plant per pot) was considered as a replicate and there were eight replicates per treatment.